Gastric toxicity of Alendronate and Methotrexate in Walker 256 carcinosarcoma jaw model

Autores

DOI:

https://doi.org/10.12662/2317-3076jhbs.v7i2.2336.p126-132.2019

Palavras-chave:

Gastric Mucosa, Carcinoma 256, Walker, Alendronate, Methotrexate

Resumo

Introduction: experimental animal models represent a key tool used to elucidate the mechanisms of action and toxicity of anticancer drugs. Objective: the purpose was to establish a correlation of neoplastic growth with the combinatorial therapeutic application of sodium alendronate (ALD) and methotrexate (MTX), and to evaluate the gastrointestinal toxicity of these drugs, in the rat Walker 256 carcinosarcoma inoculation model. Methods: female rats were selected and randomly distributed into 5 groups (n=10): negative control (NC), positive control (PC), MTX-treated group, ALD-treated group, and MTX-ALD-treated group (MTX/ALD). Tumor cells were inoculated as a suspension of 1x106cells/mL into the alveolar cavities produced by exodontia procedures. The following parameters were evaluated: body weight, tumor volume and percentage of tumor inhibition, and gastrointestinal toxicity. Results: the body weight variation was statistically significant between NC animals and PC animals, and between NC animals and ALD-treated group (p<0.01). Tumor volume variation was statistically significant between PC animals, MTX-treated group and MTX/ALD-co-treated group (p<0.05). Analysis of gastric toxicity of MTX-treated group reveled slight reduction of chief (Ch) and parietal (Pr) cellular populations; ALD-treated group exhibited gastric mucosa without histological alterations of Ch cells but intense reduction of Pr cellular population; and MTX/ALD-co-treated group presented reduction of Ch and Pr cellular populations. Conclusions: ALD does not elicit significant antitumor effects on Walker 256 carcinosarcoma cells and decreases antitumor effects of MTX due to toxicity on the gastric epithelium, which is intensified with MTX association.

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Biografia do Autor

Ana Paula Negreiros Nunes Alves, Federal University of Ceara (UFC)

DDSc, Full Professor, Department of Dental Clinic, School of Dentistry, Federal University of Ceara, Fortaleza, Ceará, Brazil

Maria Elisa Quezado Lima Verde, Federal University of Ceara

Possui graduação em Odontologia pela Universidade Federal do Ceará - UFC (2016). Possui Aperfeiçoamento em Reabilitação Oral pelo Núcleo de Excelência em Odontologia (NEXO) (2017) e em Cirurgia Oral e Estomatologia Clínica pelo Centro Universitário Christus (Unichristus) (2017). Atualmente, é aluna do Programa de Pós Graduação em Odontologia (PPGO) - UFC, em nível de Mestrado, com ênfase em Estomatopatologia, e Coordenadora Adjunta do curso de Odontologia (Centro Universitário Christus - Unichristus), além de colaborar com as atividades do laboratório de Histopatologia do Núcleo de Pesquisa e Desenvolvimento de Medicamentos - NPDM.

Paulo Goberlânio de Barros Silva, Federal University of Ceara (UFC)

DDSc Postgraduate Program in Dentistry, Department of Dental Clinic, School of Dentistry, Federal University of Ceara, Fortaleza, Ceará, Brazil

Fabrício Bitu Sousa, Federal University of Ceara (UFC)

PhD, Postgraduate Program in Dentistry, Department of Dental Clinic, School of Dentistry, Federal University of Ceara, Fortaleza, Ceará, Brazil

Mário Rogério Lima Mota, Federal University of Ceara (UFC)

PhD, Postgraduate Program in Dentistry, Department of Dental Clinic, School of Dentistry, Federal University of Ceara, Fortaleza, Ceará, Brazil

Cláudia do Ó Pessoa, Federal University of Ceara (UFC)

PhD, Full Professor, Department of Physiology and Pharmacology, School of MedicineFederal University of Ceara, Fortaleza, Ceará, Brazil;

Letícia Veras Costa Lotufo, Federal University of Ceara (UFC)

PhD, Full Professor, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceara, Fortaleza, Ceará, Brazil

Manoel Odorico de Moraes-Filho, Federal University of Ceara (UFC)

PhD, Full Professor, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceara, Fortaleza, Ceará, Brazil

Publicado

2019-04-11