Infiltration by Streptococcus mutans in provisional crowns fixed by two types of cementing agents – pilot double-blind randomized clinical trial

Ana Luiza Aquino Rego, Vanessa Anielly Cruz Moura, Janaína Câncio de Holanda Prado, Francisca Jamila Ricarte Alexandrino, Romulo Rocha Regis, Karina Matthes de Freitas Pontes

Resumo


Objective: When provisional acrylic crowns are used for a long time, they become more susceptible to marginal leakage by cariogenic bacteria. The objectives of this pilot clinical study were to compare cement based on zinc oxide-eugenol and calcium hydroxide by contamination with Streptococcus mutans, and calculate the sample size for the continuation of this study. Methods: Individuals receiving provisional crowns and following inclusion/exclusion criteria, were randomly distributed into 2 groups: zinc oxide-eugenol (n=8); calcium hydroxide (n=9). The temporary crowns were made by a blind researcher and cemented by another. Patients were also blinded by the cement used inside their crowns. After 2 months, a cement sample from the crowns’ peripheral inner face was collected, placed in a tube containing 1 mL of sterile saline, serially diluted, plated on Mitis Salivarius Bacitracin agar, and incubated for 48 hours. Colony-forming units (CFU/mL) were counted. A statistical power analysis was performed to calculate sample size (1-β=80%) and the Mann Whitney test to compare both cements (α=0.05). Results: Both cements were contaminated with S. mutans, with an average of 166.6 x 102 CFU/mL for calcium hydroxide and 435.3 x 102 CFU/mL for zinc oxide-eugenol, with no significant difference (p=0.311). The sample size calculated for this study was 36 per group. Conclusion: This pilot study suggests that there is important contamination inside provisional crowns used for two months, independent of the cement. The continuation of this study is needed, with a bigger sample size, to enable a comparison between the cements.


Palavras-chave


Interim dental prosthesis; Fixed prosthesis; Streptococcus mutans; Calcium hydroxide; Zinc oxide-eugenol cement

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DOI: http://dx.doi.org/10.12662/2317-3076jhbs.v9i1.3562.p%25p.2021

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Direitos autorais 2021 Journal of Health & Biological Sciences

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