Infiltration by Streptococcus mutans in provisional crowns fixed by two types of cementing agents – pilot double-blind randomized clinical trial

Autores

  • Ana Luiza Aquino Rego Universidade Federal do Ceará (UFC)
  • Vanessa Anielly Cruz Moura Universidade Federal do Ceará (UFC)
  • Janaína Câncio de Holanda Prado Universidade Federal do Ceará (UFC)
  • Francisca Jamila Ricarte Alexandrino Faculdade Paulo Picanço (FACPP)
  • Romulo Rocha Regis Universidade Federal do Ceará (UFC) https://orcid.org/0000-0003-2900-7815
  • Karina Matthes de Freitas Pontes Universidade Federal do Ceará (UFC) https://orcid.org/0000-0001-7071-5886

DOI:

https://doi.org/10.12662/2317-3076jhbs.v9i1.3562.p%25p.2021

Palavras-chave:

Interim dental prosthesis, Fixed prosthesis, Streptococcus mutans, Calcium hydroxide, Zinc oxide-eugenol cement

Resumo

Objective: When provisional acrylic crowns are used for a long time, they become more susceptible to marginal leakage by cariogenic bacteria. The objectives of this pilot clinical study were to compare cement based on zinc oxide-eugenol and calcium hydroxide by contamination with Streptococcus mutans, and calculate the sample size for the continuation of this study. Methods: Individuals receiving provisional crowns and following inclusion/exclusion criteria, were randomly distributed into 2 groups: zinc oxide-eugenol (n=8); calcium hydroxide (n=9). The temporary crowns were made by a blind researcher and cemented by another. Patients were also blinded by the cement used inside their crowns. After 2 months, a cement sample from the crowns’ peripheral inner face was collected, placed in a tube containing 1 mL of sterile saline, serially diluted, plated on Mitis Salivarius Bacitracin agar, and incubated for 48 hours. Colony-forming units (CFU/mL) were counted. A statistical power analysis was performed to calculate sample size (1-β=80%) and the Mann Whitney test to compare both cements (α=0.05). Results: Both cements were contaminated with S. mutans, with an average of 166.6 x 102 CFU/mL for calcium hydroxide and 435.3 x 102 CFU/mL for zinc oxide-eugenol, with no significant difference (p=0.311). The sample size calculated for this study was 36 per group. Conclusion: This pilot study suggests that there is important contamination inside provisional crowns used for two months, independent of the cement. The continuation of this study is needed, with a bigger sample size, to enable a comparison between the cements.

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Publicado

2021-06-07