Effectiveness of resveratrol as a hepatoprotector in a rat model of paracetamol-induced liver injury

Authors

  • Renan Marcel Bonilha Dezena Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil. https://orcid.org/0000-0002-9010-5724
  • Sthéfani Schiavon Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.
  • Matheus Arnosti Landi Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.
  • Gustavo Henrique da Silva Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.
  • Gisele Mara Silva Gonçalves Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil. http://orcid.org/0000-0002-3480-5777

DOI:

https://doi.org/10.12662/2317-3076jhbs.v11i1.4655.p1-9.2023

Keywords:

Resveratrol, Paracetamol, hepatoprotective activity, biodistribution, high-performance liquid chromatograph (HPLC)

Abstract

Objective: Evaluate the effectiveness of resveratrol as a hepatoprotector in a rat model of paracetamol-induced liver injury and its biodistribution to understand its pharmacokinetics. Methodology: As an experimental approach, animals were divided into the test group with 4 subgroups and the control group with 4 subgroups. Animals of the "treated" group were subjected to resveratrol pre-treatment for eight days, followed by intoxication with a high dose of paracetamol on the 8th day. Animals were euthanized to collect the blood and liver tissue samples 24 and 72 h after the last administration. Hepatoprotective activity was evaluated through serum levels of glycogen and hepatic enzymes, such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), histological and morphometric analysis of the liver tissue. For biodistribution analysis, different organs (organs, kidneys, heart and lungs) were collected and macerated, and resveratrol was quantified using high-performance liquid chromatography. Statistical analyses of morphometry, transaminases and alkaline phosphatase measurements, and biodistribution results were performed using GraphPad Prism® 3.0. Differences between groups were compared using ANOVA, followed by the Bonferroni test. Statistical significance was set at p < 0.05. 

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Author Biographies

Renan Marcel Bonilha Dezena, Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.

Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.

Sthéfani Schiavon, Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.

Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.

Matheus Arnosti Landi, Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.

Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.

Gustavo Henrique da Silva, Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.

Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.

Gisele Mara Silva Gonçalves, Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.

Department of Pharmaceutical Sciences, Pontifical Catholic University of Campinas (PUC-CAMPINAS), Campinas, SP, Brazil.

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Published

2023-10-24

How to Cite

1.
Dezena RMB, Schiavon S, Landi MA, da Silva GH, Gonçalves GMS. Effectiveness of resveratrol as a hepatoprotector in a rat model of paracetamol-induced liver injury. J Health Biol Sci. [Internet]. 2023 Oct. 24 [cited 2024 Jul. 22];11(1):1-9. Available from: https://periodicos.unichristus.edu.br/jhbs/article/view/4655

Issue

Vol. 11 No. 1 (2023): Journal of Health and Biological Sciences

Section

Original articles

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