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Authors

  • Bruna Gerardon Batista Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)
  • Pedro Alves d' Azevedo Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)

DOI:

https://doi.org/10.12662/2317-3076jhbs.v4i1.514.p5-7.2016

Keywords:

Methicillin-Resistant Staphylococcus aureus, Molecular Typing, Polymerase Chain Reaction

Abstract

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is an opportunistic pathogen that affects public health representing the most common infections related to health care and community. MRSA infections are classified as health care-associated MRSA (HA-MRSA) and community-associated MRSA (CA-MRSA) defined by the bacteria genetic profile. Objective: This study conducts a molecular characterization of MRSA isolates from a hospital in Porto Alegre, RS, Brazil, in order to determine the profile of SCCmec types. Methods: Eighty-one MRSA isolates from a hospital in Porto Alegre, RS, Brazil, collected from January to June of 2012 were included in the study. A multiplex PCR was performed to determine the SCCmec types. Results: From the 81 isolates, 24 (30%) were type I, 10 (12%) type II, 21 (26%) type III, 4 (5%) type IVa, 12 (15%) isolates were type IVc, 1 isolate was type I and IVc (1%) and 1 was type III and IVc (1%) simultaneously, while non-typable isolates corresponded to 8 isolates (10%). Conclusion: However most of the isolates were carrying SCCmec types related to HA-MRSA; the results reveal a change in the epidemiology, considering the decrease of the incidence of SCCmec type III and the increase of isolates being typed as SCCmec I.

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Author Biography

Pedro Alves d' Azevedo, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)

Possui graduação em Farmácia pela Universidade Federal do Rio Grande do Sul (1983), Especialização como Bioquímico-Análises Clínicas pela Universidade Federal do Rio Grande do Sul (1985) e em Virologia pela Universidade Federal do Rio de Janeiro (1996), Mestrado em Farmacologia pela Fundação Faculdade Federal de Ciências Médicas de Porto Alegre (1995), Doutorado em Ciências (Microbiologia Médica) pela Universidade Federal do Rio de Janeiro - UFRJ (2001) e Pós-Doutorado no Laboratório Especial de Microbiologia Clínica (LEMC) - Disciplina de Infectologia da Universidade Federal de São Paulo -UNIFESP (2007). Atualmente é Professor Associado da Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), bolsista em produtividade em pesquisa 2 do CNPq, professor doutor colaborador - Laboratório Especial de Microbiologia Clínica (UNIFESP), consultor do: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), da Fundação de Apoio a Pesquisa Científica e Tecnológica do Estado de SC e do Paraná (Fundação Araucária), da Universidade Federal de Pernambuco e da Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS). Tem, até o momento, cerca de 120 trabalhos publicados em Revistas Científicas e mais de 220 resumos apresentados em Congressos da área. Tem experiência na área de Microbiologia, com ênfase em Microbiologia Clínica/Médica, atuando principalmente nos seguintes temas: Resistência Bacteriana, Enterococcus spp, VRE, Staphylococcus spp coagulase negativos, Staphylococcus aureus, MRSA, Streptococcus spp, Pneumococo, Pseudomonas aeruginosa multiresistentes, Formação de Biofilme. Mantêm atividade de orientação, formação e aprimoramento de recursos humanos

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Published

2016-03-29

How to Cite

1.
Batista BG, Alves d’ Azevedo P. Profile. J Health Biol Sci. [Internet]. 2016 Mar. 29 [cited 2024 Sep. 28];4(1):5-7. Available from: https://periodicos.unichristus.edu.br/jhbs/article/view/514

Issue

Vol. 4 No. 1 (2016): Journal of Health and Biological Sciences

Section

Original articles

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