Characterization of the microbiota of the skin and oral cavity of Oreochromis niloticus<br>Caracterização da microbiota da pele e cavidade oral de Oreochromis niloticus<br>doi:10.12662/2317-3076jhbs.v4i3.767.p193-197.2016

Autores/as

  • Edmar Maciel Lima Junior Universidade Federal do Ceará (UFC)
  • Tereza de Jesus Pinheiro Gomes Bandeira Centro Universitário Christus (UNICHRISTUS)
  • Marcelo José Borges de Miranda Hospital São Marcos, Recife, Pernambuco, Brasil
  • Guilherme Emilio Ferreira Universitário Christus (UNICHRISTUS)
  • Ezequiel Aguiar Parente Universitário Christus (UNICHRISTUS)
  • Nelson Sarto Piccolo Chefe do Serviço de Cirurgia Plástica de Goiânia
  • Manoel Odorico de Moraes Flho Universidade Federal do Ceará (UFC)

DOI:

https://doi.org/10.12662/2317-3076jhbs.v4i3.767.p193-197.2016

Palabras clave:

Microflora, Nile tilapia, Oreochromis niloticus, Fish Farming in Brazil

Resumen

Introduction: Fish are usually exposed to higher microbial loads than land or air animals. The microbiota of fish mostly consists of Pseudomonas spp., Aeromonas spp., Shewanella putrefasciens, Acinetobacter spp. and Moraxella spp. The objective of this study was to analyze the oral cavity, and skin tissue microbiota on the Nile tilapia (Oreochromis niloticus), a fish species raised commercially in Brazil. Methods: Samples were collected from the oral cavity and skin of 20 Nile tilapia specimens (Oreochromis niloticus), each weighing approximately 1,000 grams. The samples were cultures for quantitative analysis on sheep blood agar (SBA) and chromID™ CPS® agar (CPS). Results: Eleven different bacterial species were identified on CPS and SBA plates. Gram-negative species were the most prevalent, while gram-positive Globicatella spp, Streptococcus spp and Enterococcus faecalis were also found. Pseudomonas aeruginosa species were isolated from all samples. Gram-positive Enterococcus faecalis was found in 70 and 60% of the skin and oral samples, respectively. Conclusion: For all samples studied, the microbial load was less than 100,000 CFU/g of tissue. This value is a cutoff standardized for the American Society of Microbiology to differentiate the causal agent from the colonizers. In light of this result and considering the absence of infectious signs in the fish samples, we conclude that the CFU values found in this study reflect a normal, non-infectious colonization/microbiota.

Descargas

Los datos de descargas todavía no están disponibles.

Biografía del autor/a

Tereza de Jesus Pinheiro Gomes Bandeira, Centro Universitário Christus (UNICHRISTUS)

Graduated from the Medical Course of Federal University of Ceará - UFC (1975), Master of Public Health from the UFC (2002), Specialist in Clinical Pathology Brazilian Society of Clinical Pathology (2004) and PhD in Microbiology from the UFC (2011). Professor and Coordinator of the Medical School of UNICRISTUS.

Publicado

2016-09-28

Cómo citar

1.
Lima Junior EM, Bandeira T de JPG, Miranda MJB de, Ferreira GE, Parente EA, Piccolo NS, et al. Characterization of the microbiota of the skin and oral cavity of Oreochromis niloticus<br>Caracterização da microbiota da pele e cavidade oral de Oreochromis niloticus<br>doi:10.12662/2317-3076jhbs.v4i3.767.p193-197.2016. J Health Biol Sci. [Internet]. 28 de septiembre de 2016 [citado 22 de diciembre de 2024];4(3):193-7. Disponible en: https://periodicos.unichristus.edu.br/jhbs/article/view/767